Wednesday, November 12, 2008

Feeling Dumb

Today I feel like I'm back in grad school again, just starting out. I'm setting up a PCR to run overnight, and then I'm running a gel tomorrow. Things that I know how to do, but I just spent the last five minutes asking the grad student in the lab questions like, "So, do you add ethidium bromide to the gel?" and "What percentage gel is do you usually use to run the DNA?" Earlier today, I asked deep insightful questions like, "So, how do we isolate gDNA?" and "What concentration do you usually make your primer stocks?" I can't wait until I get to the really hard part when I TA-clone and transform into E. coli. LOL.

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